The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved synteny with Medicago truncatula
Nelson, M.N., Phan, H.T.T., Ellwood, S.R., Moolhuijzen, P.M., Hane, J.K., Williams, A., O‘Lone, C.E., Fosu-Nyarko, J., Scobie, M., Cakir, M., Jones, M.G.K., Bellgard, M., Książkiewicz, M., Wolko, B., Barker, S., Oliver, R.P. and Cowling, W.A. (2006) The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved synteny with Medicago truncatula. Theoretical and Applied Genetics, 113 (2). pp. 225-238.
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We report the first gene-based linkage map of Lupinus angustifolius (narrow-leafed lupin) and its comparison to the partially sequenced genome of Medicago truncatula. The map comprises 382 loci in 20 major linkage groups, two triplets, three pairs and 11 unlinked loci and is 1,846 cM in length. The map was generated from the segregation of 163 RFLP markers, 135 gene-based PCR markers, 75 AFLP and 4 AFLP-derived SCAR markers in a mapping population of 93 recombinant inbred lines, derived from a cross between domesticated and wild-type parents. This enabled the mapping of five major genes controlling key domestication traits in L. angustifolius. Using marker sequence data, the L. angustifolius genetic map was compared to the partially completed M. truncatula genome sequence. We found evidence of conserved synteny in some regions of the genome despite the wide evolutionary distance between these legume species. We also found new evidence of widespread duplication within the L. angustifolius genome.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||Australian Centre for Necrotrophic Fungal Pathogens
School of Veterinary and Biomedical Sciences
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