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Development of Cell-Specific aptamers: Recent advances and insight into the selection procedures

Rahimizadeh, K., AlShamaileh, H., Fratini, M., Chakravarthy, M., Stephen, M., Shigdar, S. and Veedu, R. (2017) Development of Cell-Specific aptamers: Recent advances and insight into the selection procedures. Molecules, 22 (12). p. 2070.

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Abstract

Systematic evolution of ligands by exponential enrichment (SELEX) is an established procedure for developing short single-stranded nucleic acid ligands called aptamers against a target of choice. This approach has also been used for developing aptamers specific to whole cells named Cell-SELEX. Aptamers selected by Cell-SELEX have the potential to act as cell specific therapeutics, cell specific markers or cell specific drug delivery and imaging agents. However, aptamer development is a laborious and time-consuming process which is often challenging due to the requirement of frequent optimization of various steps involved in Cell-SELEX procedures. This review provides an insight into various procedures for selection, aptamer enrichment, regeneration and aptamer-binding analysis, in addition to a very recent update on all aptamers selected by Cell-SELEX procedures.

Publication Type: Journal Article
Murdoch Affiliation: Centre for Comparative Genomics
Publisher: MDPI
Copyright: © 2017 MDPI AG
URI: http://researchrepository.murdoch.edu.au/id/eprint/39809
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