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A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61β subunit

Ingley, E., Williams, J.H., Walker, C.E., Tsai, S., Colley, S., Sayer, M.S., Tilbrook, P.A., Sarna, M., Beaumont, J.G. and Klinken, S.P. (1999) A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61β subunit. FEBS Letters, 459 (1). pp. 69-74.

Free to read: https://doi.org/10.1016/S0014-5793(99)01188-6
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Abstract

We report here the isolation of a new member of the ADP-ribosylation factor (ARF)-like family (ARL-6) present in the J2E erythroleukemic cell line, but not its myeloid variants. Consistent with this lineage-restricted expression, ARL-6 mRNA increased with erythropoietin-induced maturation of J2E cells, and decreased with interleukin 6-induced differentiation of M1 monoblastoid cells. In tissues, ARL-6 mRNA was most abundant in brain and kidney. While ARL-6 protein was predominantly cytosolic, its membrane association increased following exposure to GTP-γS, like many members of the ARF/ARL family. Using the yeast two-hybrid system, six molecules which interact with ARL-6 were identified including SEC61β, a subunit of the heterotrimeric protein conducting channel SEC61p. Co-immunoprecipitation of ARL-6 confirmed a stable association between ARL-6 and SEC61β in COS cells. These results demonstrate that ARL-6, a novel member of the ADP-ribosylation factor-like family, interacts with the SEC61β subunit.

Publication Type: Journal Article
Publisher: Wiley
URI: http://researchrepository.murdoch.edu.au/id/eprint/39582
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