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Molecular biological techniques in studies of foodborne parasites

Ryan, U., Feng, Y. and Xiao, L. (2015) Molecular biological techniques in studies of foodborne parasites. In: Xiao, L., Ryan, U. and Feng, Y., (eds.) Biology of Foodborne Parasites. CRC Press, Boca Raton, pp. 21-40.

Link to Published Version: http://www.crcnetbase.com/doi/abs/10.1201/b18317-4
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Abstract

One of the most challenging issues in food safety is the detection of foodborne pathogens. Since the infectious dose of many pathogens is low, the sensitivity of the diagnostic tool becomes important. Traditionally, the detection and analysis of foodborne pathogens have relied on light or electron microscopy and culture methods. Conventional techniques such as microscopy lack sensitivity, are labor intensive, and require well-trained microscopists for accurate identification and interpretation, particularly for pathogens that are morphologically similar or very small in size or present in very low numbers. Special stains are required for the diagnosis of some pathogens. In addition, the diagnostic skills of microscopists can vary greatly from laboratory to laboratory resulting in some infections being misdiagnosed or missed completely. Furthermore, light microscopy can have a low sensitivity of detection, which is particularly relevant, considering that, for some pathogens, just a few individuals within a sample may represent an infectious dose.

Publication Type: Book Chapter
Murdoch Affiliation: School of Veterinary and Life Sciences
Publisher: CRC Press
Copyright: © 2015 Taylor & Francis Group, LLC
URI: http://researchrepository.murdoch.edu.au/id/eprint/38956
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