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Efficient expression of vip184ΔP gene under the control of promoters plus Shine-Dalgarno (SD) sequences of cry genes from Bacillus thuringiensis

Chen, J., Sun, F., Shi, Y., Xu, W., Guo, W. and Pang, Y. (2005) Efficient expression of vip184ΔP gene under the control of promoters plus Shine-Dalgarno (SD) sequences of cry genes from Bacillus thuringiensis. Journal of Applied Microbiology, 99 (2). pp. 426-434.

Link to Published Version: https://doi.org/10.1111/j.1365-2672.2005.02613.x
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Abstract

Aims:  To compare vip184ΔP gene expression time course and Vip184 protein yield under the control of promoters and Shine-Dalgarno (SD) sequences of vip184, cry3A and cry1A gene from Bacillus thuringiensis respectively.

Methods and Results:  Derived from the shuttle vector pHT3101, recombinant plasmids pHPT3, pHTP3AΔP and pHTP1AΔP were constructed with the native vip184 gene and the vip184ΔP gene, either under the control of promoters and SD sequences of cry3A or cry1A genes. When the above plasmids were transformed into an acrystalliferous B. thuringiensis strain Cry−B, their expression time course were consistent with those of vip184, cry3A and cry1A gene respectively. The maximum yields of Vip184 protein were increased when under the control of promoters plus SD sequences of cry3A and cry1A gene.

Conclusions:  The results showed that both cry3A and cry1A promoter/SD sequence combinations were able to enhance synthesis of Vip184 and change its expression time course.

Significance and Impact of the Study:  Both cry3A and cry1A promoter/SD systems offer a method for improving the expression efficacy of the vip184 gene in B. thuringiensis and it is possible to co-express the vip184 gene and cry genes and accumulate Vip184 in the form of inclusion bodies by these systems in order to construct novel useful B. thuringiensis engineered strains.

Publication Type: Journal Article
Publisher: Wiley
Copyright: 2005 The Society for Applied Microbiology
URI: http://researchrepository.murdoch.edu.au/id/eprint/37546
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