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Flow cytometric determination of oxidative burst activity of equine peripheral blood and bronchoalveolar lavage-derived leucocytes

Raidal, S.L., Bailey, G.D. and Love, D.N. (1998) Flow cytometric determination of oxidative burst activity of equine peripheral blood and bronchoalveolar lavage-derived leucocytes. The Veterinary Journal, 156 (2). pp. 117-126.

Link to Published Version: http://dx.doi.org/10.1016/S1090-0233(05)80037-1
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Abstract

Flow cytometric techniques were developed for the evaluation of oxidative burst activity in equine peripheralblood neutrophils and lymphocytes, as well as bronchoalveolar lavage derived pulmonary alveolar macrophages and lymphocytes. The oxidation of dichlorofluorescin was measured by the increased fluorescence of cells stimulated with phorbol myristate acetate or a variety of other stimulants. Flow cytometry was a suitable method for the evaluation of the intracellular oxidation in all cell populations evaluated. Analysis was rapid and cell separation before analysis was not required. Heterogenous cell populations with differinng responsiveness to phorbol myristate acetate stimulated oxidative burst were identified in peripheral blood neutrophil and alveolar macrophage populations. The current study characterizes flow cytometric techniques for the evaluation of oxidative burst activity in equine peripheral blood and bronchoalveolar lavage-derived leucocytes.

Publication Type: Journal Article
Murdoch Affiliation: School of Veterinary Studies
Publisher: Balliere Tindall
Copyright: © 1998 Baillière Tindall.
URI: http://researchrepository.murdoch.edu.au/id/eprint/36523
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