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Novel methods of improving the palatability of feeds containing praziquantel for commercially cultured yellowtail kingfish

Pilmer, Luke (2016) Novel methods of improving the palatability of feeds containing praziquantel for commercially cultured yellowtail kingfish. Honours thesis, Murdoch University.

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Abstract

Praziquantel is a broad spectrum anthelmintic drug that has been used therapeutically in humans and other animals for over 30 years. The efficacy of praziquantel against polyopisthocotylean and monopisthocotylean monogenean flukes in fish using bath treatments has been well demonstrated, however such treatments are prohibitively expensive for sea cage operations. In-feed treatments are also effective, however praziquantel is very bitter and its inclusion into fish diets has a negative impact on food intake and therefore effective dosing. This study aimed to investigate several innovative approaches to improve the palatability of feed containing praziquantel for commercially cultured yellowtail kingfish. The study revealed that freshly applying garlic extract to the surface of pellets coated with praziquantel was highly effective at increasing palatability. Incorporation of praziquantel into mash feeds before pellet extrusion, incorporation of praziquantel into hydrogenated castor oil solid lipid nanoparticles and the use of transglutaminase to strengthen the gelatin binder all proved ineffective at increasing palatability. When the garlic extract was applied 5 days prior to feeding the diets, palatability was reduced. Fish fed diets freshly coated with garlic extract consumed 100% of the ration at a praziquantel dietary inclusion level of 5 g/kg. As the praziquantel inclusion level increased to 15 g/kg the consumption reduced slightly, but not significantly. The time taken to consume the ration was significantly slower in diets containing praziquantel and garlic extract coating compared to diets without praziquantel, regardless of praziquantel dietary inclusion level. This study focused on juvenile kingfish less than 350grams. Future research should revolve around larger kingfish, testing lower concentrations of garlic extract and testing efficacy of the actual treatment in fish infested with flukes. The findings have considerable potential to increase the efficiency of application of praziquantel to yellowtail kingfish and potentially other cultured finfish.

Publication Type: Thesis (Honours)
Murdoch Affiliation: School of Veterinary and Life Sciences
Supervisor: Lymbery, Alan and Partridge, Gavin
URI: http://researchrepository.murdoch.edu.au/id/eprint/35141
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