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A simple method for monitoring chromatography column eluates for laccase activity during enzyme purification

Dekker, R.F.H., Ling, K.Y. and Barbosa, A.M. (2000) A simple method for monitoring chromatography column eluates for laccase activity during enzyme purification. Biotechnology Letters, 22 (2). pp. 105-108.

Link to Published Version: http://dx.doi.org/10.1023/A:1005680027798
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Abstract

A simple and economical method is described that allows rapid detection of laccase activity in chromatography column fractions during enzyme purification. Aliquots of column eluants are applied to filter paper coated with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) containing a numbered grid, and incubated at ambient temperature for 20 min. Indications of enzyme activity are simply observed by a colour change. This method avoids having to manually assay each fraction of a chromatographic run for enzyme activity.

Publication Type: Journal Article
Murdoch Affiliation: School of Biological Sciences and Biotechnology
Publisher: Springer Netherlands
URI: http://researchrepository.murdoch.edu.au/id/eprint/34661
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