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Isolation and growth of rhizobia

Hungria, M., O'Hara, G.W., Zilli, J.E., Araujo, R.S., Deaker, R. and Howieson, J.G. (2016) Isolation and growth of rhizobia. In: Howieson, J.G. and Dilworth, M.J., (eds.) Working with rhizobia. Australian Centre for International Agricultural Research, Canberra, pp. 39-60.

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Abstract

This chapter describes basic techniques for the isolation and growth of rhizobia, some of which have been used for more than a century. While these techniques re¬tain their importance, the success of current and future rhizobiology studies and enterprises will depend on the training, skills and techniques described in this chapter. A note of caution: nodules (particularly those collected from the field) are not always occupied by a single rhizobial isolate nor even by a single micro-or¬ganism. Nodules of pea and lupin, for example, have been described as containing both the nitrogen-fixing symbiont and associative organisms such as Micromono¬spora (Trujillo et al. 2010). Hence, we must be prepared for a range of organisms to appear on growth plates during isolation procedures. Recognition of rhizobia when growing on a solid medium is an essential skill in rhizobiology.

Publication Type: Book Chapter
Murdoch Affiliation: Centre for Rhizobium Studies
Publisher: Australian Centre for International Agricultural Research
Copyright: © Australian Centre for International Agricultural Research (ACIAR) 2016
URI: http://researchrepository.murdoch.edu.au/id/eprint/34579
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