Cobalt and nitrogen fixation in Lupinus angustifolius L. III. DNA and methionine in bacteroids
Dilworth, M.J. and Bisseling, T. (1984) Cobalt and nitrogen fixation in Lupinus angustifolius L. III. DNA and methionine in bacteroids. New Phytologist, 98 (2). pp. 311-316.
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Bacteroids from cobalt-deficient root nodules of Lupinus angustifolius L. cv. Unicrop were isolated and assayed for products of the cobalamin-dependent enzymes methionine synthetase, ribonucleotide reductase and methylmalonyl-CoA mutase.
Since bacteroids from lupin nodules did not show propionate-dependent O2 uptake, it was not possible to assess whether in vivo action of methylmalonyl-CoA mutase was affected, although it appears unlikely to be of major significance.
Cobalt deficiency decreased the DNA content per bacteroid in crown nodules, but not in later-formed lateral nodules. The percentage of amino-acid nitrogen accounted for by methionine was also lowered in bacteroids from crown nodules but not lateral nodules.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Environmental and Life Sciences|
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