A rapid (20 h) solid screening medium for detecting methicillin-resistant Staphylococcus aureus
Perry, P.L., Coombs, G.W., Boehm, J.D. and Pearman, J.W. (1998) A rapid (20 h) solid screening medium for detecting methicillin-resistant Staphylococcus aureus. Journal of Hospital Infection, 40 (1). pp. 67-72.
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Methicillin aztreonam mannitol salt agar is a sensitive and reliable solid screening medium for detecting methicillin-resistant Staphylococcus aureus (MRSA). With this medium an incubation period of only 20 h is sufficient to either produce visible colonies of MRSA or to exclude MRSA (no staphylococcal colonies). Coagulase testing (requiring a further 6 h) enables coagulase-positive isolates to be provisionally reported as 'possible MRSA' 26-30 h after the swabs were collected. The medium supports growth of intrinsically resistant staphylococci including low-expression-class MRSA (methicillin minimum inhibitory concentration (MIC) 8-16 mg/L), but methicillin susceptible staphylococci and β-lactamase hyperproducers are suppressed.
|Publication Type:||Journal Article|
|Publisher:||W.B. Saunders Ltd|
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