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Methods for reduced cost and lower sample prep volumes for genetic analysis applications

Vaezeslami, S., Tao, J., Blinco, J., Yu, F., Sinha, R., Heo, S-J, Miller, D., Watson, M., Pitcher, D., Boffelli, D., Quake, S., Weissman, I. and Jenkins, J. (2015) Methods for reduced cost and lower sample prep volumes for genetic analysis applications. In: American Society of Human Genetics (ASHG) 2015 Annual Meeting, 6 - 10 October 2015, Baltimore, MD.

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Abstract

As the cost of NGS has decreased, the library preparation cost has become a larger portion of the total expenditure. This is especially true for high-throughput applications, such as single-cell analysis. Therefore, there is a need to develop methods that can not only study the transcriptomes of single cells, but can also feasibly analyze large numbers of single cells.

Miniaturizing the sample preparation volume provides the opportunity for significant cost savings. Using TTP Labtech’s mosquito liquid handlers, reagent and sample quantities can be scaled down to picogram values.

Publication Type: Conference Item
Murdoch Affiliation: Institute for Immunology and Infectious Diseases
Conference Website: http://www.ashg.org/2015meeting/
URI: http://researchrepository.murdoch.edu.au/id/eprint/31890
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