Turnover of carbon pools labelled with [14c]glucose during in vitro culture of preimplantation mouse embryos
Pike, I.L. and Wales, R.G. (1982) Turnover of carbon pools labelled with [14c]glucose during in vitro culture of preimplantation mouse embryos. Australian Journal of Biological Sciences, 35 (6). pp. 637-644.
The pulse-chase technique was used to study the uptake and turnover of glucose carbon by mouse embryos in vitro. During a 1 h pulse the uptake of glucose into all embryonic fractions increased between the eight-celled and the morula-early blastocyst stages of development. Whilst most of the glucose carbon entered the non-glycogen, acid-soluble pool, significant amounts were isolated in acid-insoluble macromolecules and, at the later stage of development, in acid-soluble glycogen.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Veterinary Studies|
|Copyright:||© 1982 ASEG.|
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