Rapid detection of H and R Panton–Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis
Tong, S.Y.C., Lilliebridge, R.A., Holt, D.C., Coombs, G.W., Currie, B.J. and Giffard, P.M. (2010) Rapid detection of H and R Panton–Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis. Diagnostic Microbiology and Infectious Disease, 67 (4). pp. 399-401.
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We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC).
|Publication Type:||Journal Article|
|Copyright:||© 2010 Published by Elsevier Inc.|
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