Development of simple sequence repeat markers for Botryosphaeria spp. with Fusicoccum anamorphs
Slippers, B., Burgess, T., Wingfield, B.D., Crous, P.W., Coutinho, T.A. and Wingfield, M.J. (2004) Development of simple sequence repeat markers for Botryosphaeria spp. with Fusicoccum anamorphs. Molecular Ecology Notes, 4 (4). pp. 675-677.
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We report the development of eight sets of microsatellite markers for the ascomycete fungus and tree pathogen, Botryosphaeria parva. The primers were identified after cloning and sequencing of fragments amplified using simple sequence repeat (SSR) primers. Genome walking was used to determine unknown sequences on either side of new SSRs. The primers were tested and proved useful in nine other Botryosphaeria species that all have Fusicoccum anamorphs, similar to B. parva.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Biological Sciences and Biotechnology|
|Copyright:||© 2004 Blackwell Publishing Ltd|
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