Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins
Abraham, S., Chin, J., Brouwers, H.J.M., Turner, B., Zhang, R. and Chapman, T.A. (2011) Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins. Applied and Environmental Microbiology, 77 (18). pp. 6691-6693.
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Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).
|Publication Type:||Journal Article|
|Publisher:||American Society for Microbiology|
|Copyright:||© 2011, American Society for Microbiology.|
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