A convex “Ferguson plot” of polystyrene particles in electrophoresis on 0.25 to 2.0 % polyacrylamide (30 % Bis-crosslinked)
Orbán, L., Tietz, D. and Chrambach, A. (1987) A convex “Ferguson plot” of polystyrene particles in electrophoresis on 0.25 to 2.0 % polyacrylamide (30 % Bis-crosslinked). Electrophoresis, 8 (10). pp. 471-476.
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The plot of log(mobility) vs. gel concentration for polystyrene particles of 26.9 and 44.2 nm radius in 30% Bis-crosslinked polyacrylamide below 2.2 %T, containing 22.5% Metrizamide and 6 M urea, is convex. The convex plots of the two particles are essentially parallel. This parallelism suggests a retardation by a mechanism other than sieving. The parallel plots cover a range of polyacrylamide concentrations characterized by progressive fluidity as %T is lowered below 2 %T. The convex curvature renders suspect the previous free mobility and valence determinations in polyacrylamide gel electrophoresis obtained by linear extrapolation of Ferguson plots to 0 %T. Nonlinear extrapolation to 0 % T of plots of log(mobility) vs. gel concentration in the concentration range below 2 %T of 30% Bis-crosslinked polyacrylamide for the two polystyrene particles, corrected for the effects on mobilities of Metrizamide, urea and a lower ionic strength, provides free mobility values similar to those obtained under otherwise identical conditions on agarose gel electrophoresis.
|Publication Type:||Journal Article|
|Publisher:||John Wiley & Sons Ltd|
|Copyright:||© 1987 VCH Verlagsgesellschaft mbH|
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