Heat-inducible expression of a reporter gene detected by transient assay in Zebrafish
Adam, A., Bartfai, R., Lele, Z., Krone, P.H. and Orbán, L. (2000) Heat-inducible expression of a reporter gene detected by transient assay in Zebrafish. Experimental Cell Research, 256 (1). pp. 282-290.
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Heat-inducibility of two reporter constructs expressing lacZ gene under the control of mouse and Xenopus hsp70 promoters was tested in zebrafish (Danio rerio) embryos using a transient expression system. Cells expressing β-galactosidase were stained blue by histochemical staining and their average number per embryo was used as an indicator of the expression level of the reporter gene. Both constructs were heat-inducible in the embryonic tissues and showed similar heat dependence (increasing expression levels from 35–36°C up to 39°C with an apparent decrease at 40°C), resembling that of the zebrafish hsp70 genes. However, their induction kinetics were different, which might be due to differences in their 5′ UTRs. Spatial expression patterns of the two hsp/lacZ constructs and an endogenous hsp70 gene were mostly similar on the RNA level. These results indicate that our approach is applicable for in vivo analysis of the heat-shock response and that exogenous heat-shock promoters may be useful for inducible expression of transgenes in fish.
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|Copyright:||© 2000 Academic Press.|
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