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Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase

Veedu, R.N., Vester, B. and Wengel, J. (2009) Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase. Organic & Biomolecular Chemistry, 7 (7). pp. 1404-1409.

Link to Published Version: http://dx.doi.org/10.1039/B819946A
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Abstract

Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and LNA-5-methyl-CTP, were investigated as substrates for KOD DNA polymerase. The results reveal that KOD DNA polymerase is an efficient catalyst for template directed synthesis of DNA oligonucleotide duplexes containing a large number of LNA nucleotides by primer extension reactions. Furthermore, KOD DNA polymerase is shown to be suitable for the PCR amplification of an LNA-modified DNA duplex.

Publication Type: Journal Article
Publisher: Royal Society of Chemistry
Copyright: © 2009 Royal Society of Chemistry
URI: http://researchrepository.murdoch.edu.au/id/eprint/27434
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