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Cleavage and protection of locked nucleic acid–modified DNA by restriction endonucleases

Crouzier, L., Dubois, C., Wengel, J. and Veedu, R.N. (2012) Cleavage and protection of locked nucleic acid–modified DNA by restriction endonucleases. Bioorganic & Medicinal Chemistry Letters, 22 (14). pp. 4836-4838.

Link to Published Version: http://dx.doi.org/10.1016/j.bmcl.2012.05.113
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Abstract

Locked nucleic acid (LNA) is one of the most prominent nucleic acid analogues reported so far. We herein for the first time report cleavage by restriction endonuclease of LNA-modified DNA oligonucleotides. The experiments revealed that RsaI is an efficient enzyme capable of recognizing and cleaving LNA-modified DNA oligonucleotides. Furthermore, introduction of LNA nucleotides protects against cleavage by the restriction endonucleases PvuII, PstI, SacI, KpnI and EcoRI.

Publication Type: Journal Article
Publisher: Elsevier BV
Copyright: © 2012 Elsevier Ltd.
URI: http://researchrepository.murdoch.edu.au/id/eprint/27413
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