Cleavage and protection of locked nucleic acid–modified DNA by restriction endonucleases
Crouzier, L., Dubois, C., Wengel, J. and Veedu, R.N. (2012) Cleavage and protection of locked nucleic acid–modified DNA by restriction endonucleases. Bioorganic & Medicinal Chemistry Letters, 22 (14). pp. 4836-4838.
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Locked nucleic acid (LNA) is one of the most prominent nucleic acid analogues reported so far. We herein for the first time report cleavage by restriction endonuclease of LNA-modified DNA oligonucleotides. The experiments revealed that RsaI is an efficient enzyme capable of recognizing and cleaving LNA-modified DNA oligonucleotides. Furthermore, introduction of LNA nucleotides protects against cleavage by the restriction endonucleases PvuII, PstI, SacI, KpnI and EcoRI.
|Publication Type:||Journal Article|
|Copyright:||© 2012 Elsevier Ltd.|
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