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Synthesis of DNA oligonucleotides containing C5-ethynylbenzenesulfonamide-modified nucleotides (EBNA) by polymerases towards the construction of base functionalized nucleic acids

Goubet, A., Chardon, A., Kumar, P., Sharma, P.K. and Veedu, R.N. (2013) Synthesis of DNA oligonucleotides containing C5-ethynylbenzenesulfonamide-modified nucleotides (EBNA) by polymerases towards the construction of base functionalized nucleic acids. Bioorganic & Medicinal Chemistry Letters, 23 (3). pp. 761-763.

Link to Published Version: http://dx.doi.org/10.1016/j.bmcl.2012.11.096
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Abstract

C5-Ethynylbenzenesulfonamide-modified nucleotide (EBNA) was investigated as substrate of various DNA polymerases. The experiments revealed that KOD, Phusion and Klenow DNA polymerases successfully accepted EBNA-T nucleotide as a substrate and yielded the fully extended DNA. KOD DNA polymerase was found to be the most efficient enzyme to furnish EBNA-T containing DNA in good yields. Phusion DNA polymerase efficiently amplified the template containing EBNA-T nucleotides by PCR.

Publication Type: Journal Article
Publisher: Elsevier BV
Copyright: © 2012 Elsevier Ltd.
URI: http://researchrepository.murdoch.edu.au/id/eprint/27407
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