Rapid separation of seed gliadins by reversed-phase ultra performance liquid chromatography (RP-UPLC) and its application in wheat cultivar and germplasm identification
Han, C., Lu, X., Yu, Z., Li, X., Ma, W. and Yan, Y. (2015) Rapid separation of seed gliadins by reversed-phase ultra performance liquid chromatography (RP-UPLC) and its application in wheat cultivar and germplasm identification. Bioscience, Biotechnology, and Biochemistry, 79 (5). pp. 808-815.
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To separate gliadin from wheat flour, a novel and stability-indicating reversed-phase ultra performance liquid chromatography (RP-UPLC) method is established and optimized. A comparative analysis of routine capillary electrophoresis (CE), reversed-phase high-performance liquid chromatography (RP-HPLC), and RP-UPLC was performed and the results showed that the resolution and efficiency of RP-UPLC were significantly higher than those of CE and RP-HPLC. Characteristic RP-UPLC patterns of different bread wheat variety and related species were readily identified. These results demonstrated that our RP-UPLC procedure resulted in significant improvements in sensitivity, speed, and resolution, and thus is highly useful in wheat cultivar and germplasm identification.
The novel UP-UPLC method showing high resolution and efficiency in the separation of seed gliadins might be applied to wheat variety and germplasm identification.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Veterinary and Life Sciences|
|Publisher:||Taylor & Francis|
|Copyright:||© 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry|
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