Low-dose oral type I interferons reduce early virus replication of murine cytomegalovirus in vivo
Beilharz, M.W., McDonald, W., Watson, M.W., Heng, J., McGeachie, J. and Lawson, C.M. (1997) Low-dose oral type I interferons reduce early virus replication of murine cytomegalovirus in vivo. Journal of Interferon & Cytokine Research, 17 (10). pp. 625-630.
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Immunity to viral infections involves both innate and antigen-specific immune responses. The antiviral properties of interferons (IFNs) are part of the innate immune response. Low doses of type I IFNs (IFN-α and IFN-β) administered daily (10 IU per mouse) by the oral route significantly reduced the early replication of murine cytomegalovirus (MCMV) in both the spleen and liver of MCMV-infected susceptible BALB/c mice. Significant inhibition of virus replication was observed for two different inoculum doses of virus (2 x 104 pfu per mouse [0.6 LD50] and 2 x 104.12 pfu per mouse [0.8 LD50]). Analysis of IFN retention, using [35S]-labeled IFN-α1 compared with the nonreceptor binding mutant IFN-α1 (R33M) administered orally to mice, revealed binding of wild-type IFN-α1 to several tissues. In particular, IFN was retained by tissues proximal to lymphoid regions, including the posterior nasal cavity, posterior tongue, small intestine, and rectum. These findings suggest that type I IFNs may inhibit MCMV replication by distal binding of the orally administered IFN to various tissues, which in turn augment the primary immune response to virus infection.
|Publication Type:||Journal Article|
|Publisher:||Mary Ann Liebert Inc.|
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