Normalisation of gene expression in MDX mice muscle
Adams, A.M., Fletcher, S., Ly, T., Johnsen, R. and Wilton, S. (2009) Normalisation of gene expression in MDX mice muscle. In: 6th Australasian Gene Therapy Society Meeting, 29 April - 1 May 2009, Kerry Packer Education Centre. Royal Prince Alfred Hospital, Sydney, NSW.
Duchenne muscular dystrophy (DMD), a recessive X-linked form of muscular dystrophy caused by mutations in the dystrophin gene, is characterised by rapid progression of muscle degeneration, eventually leading to loss in ambulation, paralysis, and death. The mdx mouse model of muscular dystrophy has a nonsense mutation in exon 23 of the dystrophin gene. We have previously shown a morpholino antisense oligomer could remove this single exon, resulting in an in-frame mRNA transcript encoding a shortened but functional dystrophin protein. In-order to further investigate the consequences associated with this protein restoration, we use Genechip Exon Arrays to compare expression levels in the diaphragm of 10 week old treated mdx mice to that of age matched mdx and C57BL control mice. Quality control and statistical analysis were necessary to normalise microarray data. A oneway ANOVA and fold changes were used to compile gene lists. These genes were categorised in three groups,Molecular function, Biological process and Pathway, based on gene ontology classification e.g. signal transduction, ion transport etc, and then analysed in more detail. The data arising from this study will provide insight into gene interactions and their affects on pathways that are involved in muscle regeneration, which may provide additional therapies for DMD patients.
|Publication Type:||Conference Item|
|Item Control Page|