Analysis of rye B-chromosome structure using fluorescencein situ hybridization (FISH)
Wilkes, T.M., Francki, M.G., Langridge, P., Karp, A., Jones, R.N. and Forster, J.W. (1995) Analysis of rye B-chromosome structure using fluorescencein situ hybridization (FISH). Chromosome Research, 3 (8). pp. 466-472.
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Fluorescencein situ hybridization (FISH) has been used to analyse the structure of the rye B chromosome. Genomicin situ hybridization (GISH) demonstrates the high level of overall similarity between A and B chromosomes of rye, as well as the presence of a number of specific sequences. The B-specific repeat families D1100 and E3900 have been analysed in terms of their physical location and possible contiguity. Rye Bs contain members of the rye-specific dispersed repetitive family R173, as well as centromeric regions similar to those of the As. The B chromosomes analysed in our study lack detectable rDNA sequences. Anomalous results have been obtained with a number of subtelomeric repetitive probes from rye. Bs usually lack these sequences, but evidence is presented that in some cases A–B translocation events may relocate such sequences from the As to the Bs. These data are discussed in the context of current models for the origin of the B chromosome.
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|Publisher:||Kluwer Academic Publishers|
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