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Effects of copper deficiency on copper and nitrogen concentrations and enzyme activities in aerial parts of vegetative subterranean clover plants

Walker, C.D. and Loneragan, J.F. (1981) Effects of copper deficiency on copper and nitrogen concentrations and enzyme activities in aerial parts of vegetative subterranean clover plants. Annals of Botany, 48 (1). pp. 65-73.

Link to Published Version: http://aob.oxfordjournals.org/content/48/1/65
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Abstract

The relationships of copper supply to the copper and nitrogen concentrations and activities of five copper enzymes are examined in the vegetative, aerial parts of subterranean clover plants

In plants given adequate copper, copper and nitrogen concentrations were highest in shoot tips and in young and mature leaves and lowest in old leaves and in stems and petioles The activity of superoxide dismutase followed a similar pattern except that the values for stems and petioles were appreciably lower than those for old leaves The activities of three copper oxidases (ascorbate, diamine, o-diphenol) were very high in shoot tips, intermediate in young leaves and extremely low in mature and old leaves and in stems and petioles Cytochrome oxidase behaved similarly except that its activity in young leaves was as high as that in shoot tips

Copper deficiency depressed the copper concentration of shoot tips, and young and mature leaves by 35–65 per cent it depressed nitrogen concentrations in all leaves by about 20 per cent but in stems and petioles had no effect Copper deficiency depressed the activities of all enzymes in shoot tips and also those of the oxidases in young leaves by 70–95 per cent but had little or no effect in other parts

Ascorbate and cytochrome oxidase activities of young leaves from copper-deficient plants doubled after incubation in copper solutions copper-treated plants did not respond The results confirm that induction of ascorbate or cytochrome oxidase activities in leaves treated with copper could give a specific diagnostic test for copper deficiency in plants only young leaves or shoot tips should be used in enzymic assays

Publication Type: Journal Article
Murdoch Affiliation: School of Environmental and Life Sciences
Publisher: Oxford University Press
URI: http://researchrepository.murdoch.edu.au/id/eprint/21215
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