Fructose metabolism in wild-type, fructokinase-negative and revertant strains of Rhizobium leguminosarum
Glenn, A.R., Arwas, R., McKay, I.A. and Dilworth, M.J. (1984) Fructose metabolism in wild-type, fructokinase-negative and revertant strains of Rhizobium leguminosarum. Microbiology, 130 (2). pp. 231-237.
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Rhizobium leguminosarum accumulates fructose by an active process sensitive to azide, 2,4-dinitrophenol and carbonyl cyanide m-chlorophenylhydrazone. The fructose is not phosphorylated during transport. Sorbose and glucose interfere with fructose uptake. Inside the cell fructose is metabolized via fructose 6-phosphate; there is no evidence for an alternative metabolic route via sorbitol to glucose or via sorbitol 6-phosphate to fructose 6-phosphate. Tn5-induced mutants lacking fructokinase failed to grow on fructose, mannitol or sorbitol and grew slowly on sucrose; growth was normal on all other single carbon sources tested. Growth of these mutants on a range of carbon sources was retarded by added fructose. Revertants which had regained the capacity to utilize fructose all had an unstable fructokinase which could be partially stabilized by fructose.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Environmental and Life Sciences|
|Publisher:||Society for General Microbiology|
|Copyright:||© 1984 SGM|
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