Catalog Home Page

Aromatic metabolism in Rhizobium trifolii-catechol 1,2-dioxygenase

Chen, Y.P., Glenn, A.R. and Dilworth, M.J. (1985) Aromatic metabolism in Rhizobium trifolii-catechol 1,2-dioxygenase. Archives of Microbiology, 141 (3). pp. 225-228.

Link to Published Version:
*Subscription may be required


Catechol 1,2-dioxygenase has been purified 46-fold from cells of Rhizobium trifolii TA1 grown on benzoate plus glucose. The dioxygenase had a molecular weight of 107,000 and a sub-unit molecular weight of 59,000. The enzyme had a K m of 2 μM for catechol and also cleaved 4-methylcatechol. The dioxygenase contained 2 g atoms of Fe3+ per mole of enzyme which could be removed by treatment with 1,10-phenanthroline, resulting in a complete loss of activity; reactivation of the enzyme occurred specifically with Fe3+.

Publication Type: Journal Article
Publisher: Springer Verlag
Item Control Page Item Control Page