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The use of activities of carbon catabolic enzymes as a probe for the carbon nutrition of Snakebean nodule bacteroids

Saroso, S., Dilworth, M.J. and Glenn, A.R. (1986) The use of activities of carbon catabolic enzymes as a probe for the carbon nutrition of Snakebean nodule bacteroids. Microbiology, 132 (2). pp. 243-249.

Link to Published Version: http://dx.doi.org/10.1099/00221287-132-2-243
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Abstract

Summary: In sugar-grown cells of cowpea Rhizobium strain NGR234 activities for enzymes of the Entner-Doudoroff and pentose phosphate pathways were present while the virtual absence of phospho-fructokinase and fructose-bisphosphate aldolase indicated that the Embden–Meyerhof–Parnas pathway was unlikely to be significant. Invertase, fructokinase, glucose-6-phosphate dehydrogenase and the Entner–Doudoroff enzymes were present at only low activities in succinate grown cells, but were induced in sugar-grown cells. Isolated snakebean bacteroids contained very low activities of these four enzymes. Although C4-dicarboxylic acids exerted some repressive effect on induction of these enzymes, there was substantial enzyme activity induced in cells grown on sucrose plus a C4 dicarboxylic acid. The data suggest that the peribacteroid membrane may be relatively impermeable to sugars and so dictate the carbon source(s) available to the bacteroids.

Publication Type: Journal Article
Murdoch Affiliation: School of Environmental and Life Sciences
Publisher: Society for General Microbiology
URI: http://researchrepository.murdoch.edu.au/id/eprint/20703
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