Carbon catabolism in continuous cultures and bacteroids of Rhizobium leguminosarum MNF 3841
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Bacteroids of R. leguminosarum MNF3841 isolated from pea nodules using Percoll gradients had activities of TCA cycle enzymes up to 6-fold higher than those measured in free-living cells grown on fumarate or sucrose. Activities of sugar catabolic enzymes on the other hand were 2-14-fold lower in isolated bacteroids than in sucrose-grown free-living cells. In continuous culture, cells of strain MNF3841 grown on sucrose under Pi limitation had 2-3-fold higher activities of invertase, glucose-6-phosphate dehydrogenase, the Entner-Doudoroff enzymes and 6-phosphogluconate dehydrogenase, than cells grown on fumarate. With one exception O2 limited cultures had similar activities of the carbon catabolic enzymes to Pi-limited cultures grown in the same substrate. Glucose-6-phosphate dehydrogenase in O2-limited cells grown of fumarate was 50% lower than in Pi-limited cells. Co-utilization of fumarate and sucrose occurred with chemostat cultures supplied with both under a variety of conditions.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Biological and Environmental Sciences|
|Copyright:||© 1989 Springer-Verlag|
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