Genetically engineered virus disease resistance in lupins
Wylie, S., Li, H., Hoffmann, K., Ryan, K., Yang, R., Li, D., Liu, L., Buirchell, B., Jones, R.A.C. and Jones, M. (1998) Genetically engineered virus disease resistance in lupins. In: Crop Updates 1998: Lupins, 18 - 20 February 1998, Rendezvous Observation City Hotel, Scarborough, Perth
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We have developed a number of resistance genes to confer resistance to bean yellow mosaic virus and cucumber mosaic virus. The bean yellow mosaic virus genes have been transferred to Lupinus angustifolius and L. luteus and the CMV genes are presently being introduced to L. angustifolius. In the 1997 season eight transgenic L. luteus lines containing BYMV resistance genes were challenged with the virus. Two lines were shown to exhibit reduced disease symptoms, and delayed accumulation of BYMV particles after manual and aphid inoculation of the virus. Virus challenge was carried out in glasshouse trials at Murdoch University and in a screen house at the UWA Field Station. Inoculation trials will proceed again with other transgenic lines in the next growing season. We began a search for the CMV resistance gene present in L. luteus Wodjil in order to isolate and characterise it. It is hoped that in the future this gene may be transferred to other CMV susceptible legumes.
|Publication Type:||Conference Paper|
|Murdoch Affiliation:||Western Australian State Agricultural Biotechnology Centre|
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