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Use of RAPD-PCR to isolate a species specific DNA probe for Phytophthora cinnamomi

Dobrowolski, M.P. and O'Brien, P.A. (1993) Use of RAPD-PCR to isolate a species specific DNA probe for Phytophthora cinnamomi. FEMS Microbiology Letters, 113 (1). pp. 43-47.

Link to Published Version: http://dx.doi.org/10.1016/0378-1097(93)90096-K
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Abstract

The products of RAPD-PCR amplification of Phytophthora cinnamomi DNA were separated by electrophoresis in agarose. Parallel Southern blots of the gels were hybridized with nick translated DNA from different species of Phytophthora. Fragments that hybridized specifically to P. cinnamomi DNA were identified. These fragments were purified and cloned into pUC18. Their specificity for P. cinnamomi was confirmed.

Publication Type: Journal Article
Murdoch Affiliation: School of Biological and Environmental Sciences
Publisher: Blackwell Publishing
URI: http://researchrepository.murdoch.edu.au/id/eprint/19603
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