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Gene discovery and pre-breeding for Russian wheat aphid and Ug99 resistance in wheat

Cakir, M., Phan, H., Vitou, J., Haley, S., Peairs, F., Mornhinweg, D., Bohssini, M., Ogbonnaya, F., Lage, J., Tolmay, V., Malinga, J., Edwards, O., Christopher, M., Castro, A.M., Kuchel, H., Jacobs, B., Barclay, I. and Sheppard, J. (2010) Gene discovery and pre-breeding for Russian wheat aphid and Ug99 resistance in wheat. In: 3rd International Conference of Plant Molecular Breeding, 5 - 9 September 2010, Beijing, China

Abstract

The Russian Wheat Aphid (RWA) (Diuraphis noxia) is one of the major insect species that can cause economic yield losses to wheat (Triticum aestivum) and barley (Hordeum vulgare) growers around the world. This aphid, although is not yet present in Australia, is extremely damaging with up to 70-100% yield loses in wheat producing lands in various parts of the world, causing significant financial losses to the grains farmers. The objectives of the study are to: 1) Identify RWA resistant wheat germplasm from around the world and characterize against available RWA biotypes in various countries, 2) identify molecular markers closely linked to new resistance genes, and 3) introgress RWA resistance into more adapted wheat backgrounds from Australia, Kenya, where possible including Ug99 resistance into introgressions. We have evaluated a wide range of wheat lines from various parts of the world against a number of RWA biotypes from Argentina, Mexico, Hungary, South Africa, France and USA. Lines with moderate to good levels of resistance were identified. To date, with the use of F2 and DH wheat populations we have mapped genes from at least four new sources for RWA resistance on chromosomes 1DS and 7D, and closely linked SSR and DArT markers have been identified. Introgression of RWA resistance genes to adapted wheat and barley lines have progressed to BC2 generation. As we have identified some wheat lines that are resistant to both RWA and Ug99 in Kenya, four-way crosses were established to introgress resistance genes to both pests. Progress of introgressing Ug99 resistance along with RWA resistance in Kenya will further be discussed.Diuraphis noxia) is one of the major insect species that can cause economic yield losses to wheat (Triticum aestivum) and barley (Hordeum vulgare) growers around the world. This aphid, although is not yet present in Australia, is extremely damaging with up to 70-100% yield loses in wheat producing lands in various parts of the world, causing significant financial losses to the grains farmers. The objectives of the study are to: 1) Identify RWA resistant wheat germplasm from around the world and characterize against available RWA biotypes in various countries, 2) identify molecular markers closely linked to new resistance genes, and 3) introgress RWA resistance into more adapted wheat backgrounds from Australia, Kenya, where possible including Ug99 resistance into introgressions. We have evaluated a wide range of wheat lines from various parts of the world against a number of RWA biotypes from Argentina, Mexico, Hungary, South Africa, France and USA. Lines with moderate to good levels of resistance were identified. To date, with the use of F2 and DH wheat populations we have mapped genes from at least four new sources for RWA resistance on chromosomes 1DS and 7D, and closely linked SSR and DArT markers have been identified. Introgression of RWA resistance genes to adapted wheat and barley lines have progressed to BC2 generation. As we have identified some wheat lines that are resistant to both RWA and Ug99 in Kenya, four-way crosses were established to introgress resistance genes to both pests. Progress of introgressing Ug99 resistance along with RWA resistance in Kenya will further be discussed.progressed to BC2 generation. As we have identified some wheat lines that are resistant to both RWA and Ug99 in Kenya, four-way crosses were established to introgress resistance genes to both pests. Progress of introgressing Ug99 resistance along with RWA resistance in Kenya will further be discussed.

Publication Type: Conference Paper
Murdoch Affiliation: Western Australian State Agricultural Biotechnology Centre
URI: http://researchrepository.murdoch.edu.au/id/eprint/19278
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