Genetic diversity in cocoyam as revealed by random amplified polymorphic DNA
Danquah, E.Y., Blay, E.T., Offei, S.K., Fosu-Nyarko, J. and Amiteye, S. (2001) Genetic diversity in cocoyam as revealed by random amplified polymorphic DNA. African Journal of Root and Tuber Crops, 4 (2). pp. 41-46.
Forty-eight accessions of cocoyam (Xanthosoma sagittifolium) were evaluated for genetic diversity using random amplified polymorphic DNA (RAPD) analysis. Out of 50 primers screened, 10 were able to amplify the genomic DNA giving reproducible amplification patterns with individual fragments that stained intensely. Two of the primers OPA-05 and OPE-09 were considered highly informative because they amplified one or more polymorphic bands that distinguished between accessions. RAPDs showed higher genetic diversity in the accessions from Kade than those from Bunso, where the nation’s germplasm is maintained. UPGMA cluster analysis of genetic similarity estimates (Jaccard’s coefficient), separated the accessions into 5 main groups, 2 of which were one member groups. The primers will be useful for further genetic analysis and provide cocoyam breeders with suitable genetic marters for the selection of parents for crop improvement and germplasm conservation. Polymorphic markers identified in the DNA fingerprinting will also be useful in the study of mutant populations, which are currently being studied in our laboratory.
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