Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts
Laczka, O., Skillman, L., Ditcham, W.G.F., Hamdorf, B., Wong, D.K.Y., Bergquist, P. and Sunna, A. (2013) Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts. Journal of Microbiological Methods, 95 (2). pp. 182-185.
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We report a novel electrochemical method for the rapid detection of the parasitic protozoan, Cryptosporidium parvum. An antibody-based capture format was transferred onto screen-printed electrodes and the presence of horseradish peroxidase-labelled antibodies binding to the oocysts was potentiometrically detected. This method allowed the detection of 5×102 Cryptosporidium oocysts per mL in 60min.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Veterinary and Life Sciences|
|Copyright:||© 2013 Elsevier B.V.|
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