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Genetic maps of Phytophthora cinnamomi based on microsatellite fragment length polymorphisms(MFLP)

Dobrowolski, M.P., Hardy, G.E.St.J., Tommerup, I.C., Shearer, B.L., Colquhoun, I.J. and O'Brien, P.A. (2003) Genetic maps of Phytophthora cinnamomi based on microsatellite fragment length polymorphisms(MFLP). In: 8th International Congress of Plant Pathology: Solving problems in the real world, (ICPP 2003), 2 - 7 February, Christchurch; New Zealand.

Abstract

Phytophthora cinnamomi is a major oomycete pathogen of an extensive range of mainly woody plants. We conducted genetic mapping of P cinnamomi to localise genes involved in pathogenicity. Presented here are the genetic maps of five P. cinnamomi isolates developed from our analysis of four outbred mapping populations. The mapping populations were constructed using a common Al parent and four different A2 parents. Because analysis has been restricted to mapping populations of F1 hybrid progeny in this diploid species, we chose to use microsatellite fragment length polymorphisms (MLFP) as genetic markers. This technique combines the high polymorphism and codominance of microsatellites with the AFLP technique and needs no prior sequence information. The analysis yielded between one and four informative loci per primer combination (equivalent to one AFLP gel run). These genetic maps will be applied to QTL mapping of pathogenicity characters in the P. cinnamomi genome.

Publication Type: Conference Item
Murdoch Affiliation: School of Biological Sciences and Biotechnology
Publisher: Horticulture Australia
URI: http://researchrepository.murdoch.edu.au/id/eprint/18018
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