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Identification of a ribonuclease H gene in both Mycoplasma genitalium and Mycoplasma pneumoniae by a new method for exhaustive identification of ORFs in the complete genome sequences

Bellgard, M.I. and Gojobori, T. (1999) Identification of a ribonuclease H gene in both Mycoplasma genitalium and Mycoplasma pneumoniae by a new method for exhaustive identification of ORFs in the complete genome sequences. FEBS Letters, 445 (1). pp. 6-8.

Free to read: http://dx.doi.org/10.1016/S0014-5793(99)00075-7
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Abstract

Exhaustive identification of open reading frames in complete genome sequences is a difficult task. It is possible that important genes are missed. In our efforts to reanalyze the intergenic regions of Mycoplasma genitalium and Mycoplasma pneumoniae, we have newly identified a number of new open reading frames (ORFs) in both M. genitalium and M. pneumoniae. The most significant identification was that of a ribonuclease H enzyme in both species which until now has not been identified or assumed absent and interpreted as such. In this paper we discuss the biological importance of RNase H and its evolutionary implication. We also stress the usefulness of our method for identifying new ORFs by reanalyzing intergenic regions of existing ORFs in complete genome sequences.

Publication Type: Journal Article
Murdoch Affiliation: School of Information Technology
Publisher: Elsevier
Copyright: 1999 Federation of European Biochemical Societies
URI: http://researchrepository.murdoch.edu.au/id/eprint/17313
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