Rhizogenesis and root growth of Carica papaya L.in vitro in relation to auxin sensitive phases and use of riboflavin
Drew, R.A., McComb, J.A. and Considine, J.A. (1993) Rhizogenesis and root growth of Carica papaya L.in vitro in relation to auxin sensitive phases and use of riboflavin. Plant Cell, Tissue and Organ Culture, 33 (1). pp. 1-7.
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High rooting percentages and high-quality adventitious root systems for papaya (Carica papaya L.) were obtained in vitro by appropriate auxin source, duration of exposure to auxin and use of riboflavin. Root initiation of papaya shoots was higher using IBA than IAA, NAA or PCPA. Maximum rooting percentage (96%) was achieved by exposure of shoots to a medium containing 10 μM IBA for 3 days before transfer to a hormone-free medium. However, the resultant plants had small shoots and callused roots. Shoot and root growth were improved when shoots were transferred after 2 days from medium containing 10 μM IBA to hormone-free medium containing 10 μM riboflavin. Good root initiation, and root and shoot growth were also obtained when shoots were incubated for 2 days in darkness on a medium containing 10 μM IBA and 31 μM riboflavin before transfer to light. Alternatively, cultures could be placed in the light on medium containing 10 μM IBA, and after 1 day the medium overlaid with 300 μM riboflavin (1 ml over 10 ml of medium).
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Biological and Environmental Sciences|
|Publisher:||Kluwer Academic Publishers|
|Copyright:||© 1993 Kluwer Academic Publishers.|
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