Stimulation of synthesis and release of swainosonine from transformed roots of Swainsona galegifolia
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Transformed root cultures of Swainsona galegifolia were established for swainsonine production. Stimulation of production of swainsonine and its release into the culture medium was achieved using copper sulphate, reduction of medium pH and supply of swainsonine precursors. The yield of control cultures (0.3 g of roots grown in 15 ml medium for 30 days) was 79 μg swainsonine with only a trace of swainsonine in the medium. After treatment with 1 mM copper sulphate for two days before harvest on day 30, 155 μg of swainsonine was produced, of which 14.3 μg was in the medium. Reduction of medium pH from 5.7 to 2.7 for one day before harvest resulted in 159 μg of swainsonine (47 μg in the medium). Supplementation with 2 mM malonic acid for 12 days before harvest resulted in 187 μg total swainsonine (34 μg in the medium), while 2 mM pipecolic acid for six days before harvest gave the highest swainsonine yield (220 μg total, 43 μg in the medium). The increased yields were achieved through small increases in biomass, as well as increases in the level of swainsonine synthesis.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Biological and Environmental Sciences|
|Copyright:||© 1994 Published by Elsevier Ltd.|
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