Development of gene-specific markers for acid soil/aluminium tolerance in barley (Hordeum vulgare L.)
Bian, M., Waters, I., Broughton, S., Zhang, X-Q, Zhou, M., Lance, R., Sun, D. and Li, C. (2013) Development of gene-specific markers for acid soil/aluminium tolerance in barley (Hordeum vulgare L.). Molecular Breeding, 32 (1). pp. 155-164.
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Acid soil/aluminium toxicity is one of the major constraints on barley production around the world. Genetic improvement is the best solution and molecular-marker-assisted selection has proved to be an efficient tool for developing barley cultivars with acid soil/aluminium tolerance. In this study, barley variety Svanhals-introduced from CYMMIT (International Maize and Wheat Improvement Center)-was identified as acid soil/aluminium tolerant and the tolerance was mapped to chromosome 4H in 119 doubled haploid (DH) lines from a cross of Hamelin/Svanhals. The HvMATE gene, encoding an aluminium-activated citrate transporter, was selected as a candidate gene and gene-specific molecular markers were developed to detect acid soil/aluminium tolerance based on the polymerase chain reaction. Sequence analysis of the HvMATE gene identified a 21-bp indel (insertion-deletion) between the tolerant and sensitive cultivars. The new marker was further mapped to the QTL (quantitative trait loci) region on chromosome 4H for acid soil tolerance and accounted for 66.9 % of phenotypic variation in the DH population. Furthermore, the polymorphism was confirmed in other tolerant varieties which have been widely used as a source of acid soil tolerance in Australian barley breeding programs. The new gene-specific molecular marker provides an effective and simple molecular tool for selecting the acid soil tolerance gene from multiple tolerance sources.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||Western Australian State Agricultural Biotechnology Centre|
|Publisher:||Kluwer Academic Publishers|
|Copyright:||© 2013 Springer Science+Business Media Dordrecht|
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