Maceration of plant tissue by fungi is inhibited by recombinant antipectinase antibodies
Manatunga, V., Sanati, H., Tan, P. and O’Brien, P.A. (2005) Maceration of plant tissue by fungi is inhibited by recombinant antipectinase antibodies. European Journal of Plant Pathology, 112 (3). pp. 211-220.
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Polyclonal antiserum from mice immunized with extracellular proteins from Rhizoctonia solani inhibited pectinase and cellulase activities in cell free culture supernatants of Rhizoctonia solani. Spleen mRNA from these mice was used to construct a cDNA library from which antipectinase ScFv antibodies were isolated using phage display techniques. Soluble ScFv antibodies produced by individual clones in Escherichia coli inhibited polygalacturonase in the culture supernatants of a range of fungal pathogens, including ascomycetes, basidiomycetes, and oomycetes. The soluble antibodies also inhibited maceration of potato tissue by these pathogens.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Biological Sciences and Biotechnology|
|Publisher:||Kluwer Academic Publishers|
|Copyright:||© Springer 2005|
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