A major QTL controlling adult plant resistance for barley leaf rust
Li, C., Gupta, S., Zhang, X-Q, Westcott, S., Yang, J., Park, R., Platz, G., Loughman, R. and Lance, R. (2012) A major QTL controlling adult plant resistance for barley leaf rust. In: Zhang, G., Li, C. and Liu, X., (eds.) Advance in Barley Sciences. Proceedings of 11th International Barley Genetics Symposium. Springer, pp. 285-300.
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Race-specific resistance genes (Rph) for leaf rust (Puccinia hordei) are often overcome by new pathotypes with matching virulence. Adult plant resistance (APR) is considered potentially more durable for controlling barley leaf rust. Previous studies established that the cultivar Pompadour carried APR to leaf rust. A doubled haploid population (DH) of 200 lines developed from a cross Pompadour/Stirling, and the parents were phenotyped for leaf rust resistance at five field experimental sites in three agricultural zones in Australia. Using a linkage map of SSR and DArT molecular markers, a major QTL associated with the leaf rust resistance was identified on the short arm of chromosome 5H. This QTL explained between 31% and 86% of the phenotypic variation for the APR at different sites. A PCR-based molecular marker was developed and mapped at 1.6 cM to the APR gene. The present study provides new genetic material and a molecular tool for breeding new varieties with adult plant leaf rust resistance using marker-assisted selection.
|Publication Type:||Book Chapter|
|Murdoch Affiliation:||Western Australian State Agricultural Biotechnology Centre|
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