Isolation and characterization of EST-derived microsatellite loci from the fungal wheat pathogen Phaeosphaeria nodorum
Stukenbrock, E.H., Banke, S., Zala, M., McDonald, B.A. and Oliver, R.P. (2005) Isolation and characterization of EST-derived microsatellite loci from the fungal wheat pathogen Phaeosphaeria nodorum. Molecular Ecology Notes, 5 (4). pp. 931-933.
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Eleven polymorphic microsatellite loci and one minisatellite locus originating from expressed sequence tag (EST) libraries of Phaeosphaeria (syn. Stagonospora) nodorum were isolated and characterized. The satellite markers were used to genotype isolates from field populations collected in China, North America and South Africa. The number of alleles per locus ranged from two to 15. Genotype diversity ranged from 87.5 to 95.3 and gene diversity from 0.1 to 0.8. The variable levels of polymorphism within and among populations of P. nodorum renders these 12 satellite loci ideal markers for population genetic analysis of P. nodorum.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||Australian Centre for Necrotrophic Fungal Pathogens|
|Copyright:||© 2005 Blackwell Publishing Ltd.|
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