Variations and classification of toxic epitopes related to celiac disease among α-gliadin genes from fourAegilopsgenomes
Li, J., Wang, S., Li, S., Ge, P., Li, X., Ma, W., Zeller, F.J., Hsam, S.L.K., Yan, Y. and Scoles, G. (2012) Variations and classification of toxic epitopes related to celiac disease among α-gliadin genes from fourAegilopsgenomes. Genome, 55 (7). pp. 513-521.
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The α-gliadins are associated with human celiac disease. A total of 23 noninterrupted full open reading frame α-gliadin genes and 19 pseudogenes were cloned and sequenced from C, M, N, and U genomes of four diploid Aegilops species. Sequence comparison of α-gliadin genes from Aegilops and Triticum species demonstrated an existence of extensive allelic variations in Gli-2 loci of the four Aegilops genomes. Specific structural features were found including the compositions and variations of two polyglutamine domains (QI and QII) and four T cell stimulatory toxic epitopes. The mean numbers of glutamine residues in the QI domain in C and N genomes and the QII domain in C, N, and U genomes were much higher than those in Triticum genomes, and the QI domain in C and N genomes and the QII domain in C, M, N, and U genomes displayed greater length variations. Interestingly, the types and numbers of four T cell stimulatory toxic epitopes in α-gliadins from the four Aegilops genomes were significantly less than those from Triticum A, B, D, and their progenitor genomes. Relationships between the structural variations of the two polyglutamine domains and the distributions of four T cell stimulatory toxic epitopes were found, resulting in the α-gliadin genes from the Aegilops and Triticum genomes to be classified into three groups.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||Centre for Comparative Genomics|
|Publisher:||NRC Research Press|
|Copyright:||© 2012 Published by NRC Research Press|
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