Catalog Home Page

PCR amplification from fixed tissue indicates frequent involvement of Brachyspira aalborgi in human intestinal spirochetosis

Mikosza, A.S.J., La, T., Brooke, C.J., Lindboe, C.F., Ward, P.B., Heine, R.G., Guccion, J.G., de Boer, W.B. and Hampson, D.J. (1999) PCR amplification from fixed tissue indicates frequent involvement of Brachyspira aalborgi in human intestinal spirochetosis. Journal of Clinical Microbiology, 37 (6). pp. 2093-2098.

[img]
Preview
PDF - Published Version
Download (160kB) | Preview
    Link to Published Version: http://jcm.asm.org/content/37/6/2093.abstract
    *Subscription may be required

    Abstract

    PCR procedures amplifying portions of the 16S rRNA and NADH oxidase genes of Brachyspira aalborgi and Serpulina pilosicoli were applied to DNA extracted from paraffin-embedded human colonic or rectal tissues from 30 Norwegian, Australian, and U.S. patients, 16 of whom had histologic evidence of intestinal spirochetosis (IS). B. aalborgi-specific sequences were identified by PCR in 10 of the IS patients (62.5%) but none of the others, while S. pilosicoli sequences were not detected in tissues from any patient. Direct sequencing of products from three of the positive samples provided further confirmation of the presence of B. aalborgi. B. aalborgi may be a more common cause of intestinal spirochetosis than has been previously thought.

    Publication Type: Journal Article
    Murdoch Affiliation: School of Veterinary and Biomedical Sciences
    Publisher: American Society for Microbiology
    Copyright: © 1999 American Society for Microbiology
    URI: http://researchrepository.murdoch.edu.au/id/eprint/9760
    Item Control Page

    Downloads

    Downloads per month over past year