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HLA-B*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay

Hammond, E., Mamotte, C., Nolan, D. and Mallal, S. (2007) HLA-B*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay. Tissue Antigens, 70 (1). pp. 58-61.

Link to Published Version: http://dx.doi.org/10.1111/j.1399-0039.2007.00840.x
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Abstract

Inheritance of HLA-B*5701 is a strong predictor of a hypersensitivity reaction to the anti-HIV drug abacavir. The identification of susceptible individuals prior to the institution of abacavir therapy is therefore of clinical importance and has generated demand for a simple and rapid diagnostic test for carriage of HLA-B*5701. In this study, we describe the development of such a method based on allele-specific polymerase chain reaction (AS-PCR) and melting curve analysis. Ninety-six patient samples including 36 HLA-B*5701-positive samples and 60 HLA-B*5701-negative samples were analysed. Compared with sequence-based typing, this method had 100% sensitivity and specificity for the HLA-B*5701 allele. In conclusion, the AS-PCR/melting curve approach minimises post-polymerase chain reaction handling processing and provides an attractive alternative to currently described AS-PCR methods.

Publication Type: Journal Article
Murdoch Affiliation: Centre for Clinical Immunology and Biomedical Statistics
Publisher: Blackwell Publishing
Copyright: © 2007 Blackwell Munksgaard.
URI: http://researchrepository.murdoch.edu.au/id/eprint/9125
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