Catalog Home Page

Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential

Parkar, U., Traub, R.J., Kumar, S., Mungthin, M., Vitali, S., Leelayoova, S., Morris, K. and Thompson, R.C.A. (2007) Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential. Parasitology, 134 (3). pp. 359-367.

[img]
Preview
PDF - Published Version
Download (93kB) | Preview
    Link to Published Version: http://dx.doi.org/10.1017/S0031182006001582
    *Subscription may be required

    Abstract

    In vitro propagation followed by PCR, and a PCR-based method capable of the direct detection of Blastocystis in faeces were utilized to detect Blastocystis from various hosts in Australia, including primates and their handlers from the Perth Zoo. In addition, Blastocystis isolates from dogs and humans living in a localized endemic community in Thailand were also characterized genetically. PCR-based detection directly from faeces was shown to be more sensitive compared with in vitro culture for the detection of Blastocystis. Moreover, phylogenetic analysis of Blastocystis isolates amplified utilizing in vitro techniques prior to PCR revealed that this method favoured the preferential amplification of Blastocystis subtype 5 over subtype 1. This study is the first to provide molecular-based evidence supporting the zoonotic potential of Blastocystis in dogs, possums and primates in a natural setting.

    Publication Type: Journal Article
    Murdoch Affiliation: School of Veterinary and Biomedical Sciences
    Publisher: Cambridge University Press
    Copyright: © 2006 Cambridge University Press.
    URI: http://researchrepository.murdoch.edu.au/id/eprint/9035
    Item Control Page

    Downloads

    Downloads per month over past year