Evaluation of the class II region of the major histocompatibility complex of the greyhound with the genomic matching technique and sequence-based typing

Fliegner, R.A., Holloway, S.A., Lester, S., McLure, C.A. and Dawkins, R.L. (2008) Evaluation of the class II region of the major histocompatibility complex of the greyhound with the genomic matching technique and sequence-based typing. Tissue Antigens, 72 (2). pp. 131-136.

Abstract

The class II region of the major histocompatibility complex was evaluated in 25 greyhounds by sequence-based typing and the genomic matching technique (GMT). Two new DLA-DRB1 alleles were identified. Twenty-four dogs carried the DLA-DRB1*01201/DQA1*00401/DQB1*01303/DQB1*01701 haplotype, which carries two DQB1 alleles. One haplotype was identified from which DQB1 and DQA1 appeared to be deleted. The GMT enabled detection of DQB1 copy number, discrimination of the different class II haplotypes and the identification of new, possibly biologically relevant polymorphisms.