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Starch characterisation and variability in GBSS loci of synthetic hexaploid wheats and their durum and Aegilops tauschii parents

Konik-Rose, C.M., Rahman, S., Appels, R., Moss, R., McMaster, G., Marshall, D.R. and Stoddard, F.L. (2009) Starch characterisation and variability in GBSS loci of synthetic hexaploid wheats and their durum and Aegilops tauschii parents. Euphytica, 167 (2). pp. 203-216.

Link to Published Version: http://dx.doi.org/10.1007/s10681-008-9864-5
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Abstract

Greater variability in starch properties is found in lower ploidy wheats than in commercial hexaploid wheats. This paper reports on the starch properties and variability in granule bound starch synthase (GBSS) loci of 17 diploid (Aegilops tauschii) and 12 tetraploid (durums) potential progenitors of wheat, compared with 29 synthetic hexaploid wheats produced from such progenitors. Starch properties examined were granule size distribution, swelling power, amylose content, gelatinisation and amylose-lipid dissociation properties. A PCR screening method was able to detect the presence or absence of each of the three GBSS genes. It also detected polymorphisms in eight diploids and nine hexaploids, all displaying the same 25 bases deletion in the D genome allele of GBSS. Two tetraploids and five hexaploids were null 4A for GBSS. There was little difference in the amylose contents and amylose-lipid dissociation peak temperatures of the synthetic hexaploids and the lower ploidy wheats. The synthetic hexaploids showed intermediate swelling power values with the durums giving the highest swelling powers. The durums also had higher B granule contents than the A. tauschii accessions, but not as high as the synthetics. However, the A. tauschii samples gave the highest gelatinisation peak temperatures. The presence of the null 4A mutation was positively correlated with swelling power, amylose content and DSC measurements. The new smaller D genome allele of GBSS was associated with slightly higher swelling power. These results confirm the value of wheat progenitor lines as sources of new starch properties for hexaploid wheat.

Publication Type: Journal Article
Murdoch Affiliation: Centre for Comparative Genomics
Publisher: Kluwer Academic Publishers
Copyright: © 2008 Springer Science+Business Media B.V.
URI: http://researchrepository.murdoch.edu.au/id/eprint/7816
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