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Transformation of Phytophthora cinnamomi with the green Fluorescent Protein (GFP) for studies on survival

McCarren, K.L., McComb, J.A., Shearer, B., O'Brien, P. and Hardy, G.E.St.J. (2005) Transformation of Phytophthora cinnamomi with the green Fluorescent Protein (GFP) for studies on survival. In: 15th Australasian Plant Pathology Society Conference, 26-29 September 2005 , Geelong, Vic..

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    Abstract

    For soilborne plant pathogens such as Phytophthora cinnamomi, differentiating between spores of P. cinnamomi and other similar microorganisms can be challenging, making it difficult to determine the form the pathogen survives in soils and host tissue. Previous research has shown that the pathogen may be capable of producing thick-walled chlamydospores (1). However, due to the difficulty in germinating these structures routinely, presumably due to dormancy, research has not been able to progress in this area as it is unclear whether these are actually propagules of P. cinnamomi.

    The transformation of P. cinnamomi with the Green Fluorescent Protein (OFP) provides the opportunity to study the growth and survival in plants and soil under non-sterile conditions.

    The present study aimed to transform P. cinnamomi using the protoplast method to incorporate the GFP and geneticin resistance genes. The stability and pathogenicity of transformants were then compared with non-transformed isolates before studying formation and survival of chlamydospores under sterile and non-sterile conditions.

    Publication Type: Conference Item
    Murdoch Affiliation: Centre for Phytophthora Science and Management
    School of Biological Sciences and Biotechnology
    URI: http://researchrepository.murdoch.edu.au/id/eprint/7670
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